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| Destoumieux, Delphine; Bulet, Philippe; Loew, Damarys; Van Dorsselaer, Alain; Rodriguez, Jenny; Bachere, Evelyne. |
| We report here the isolation of three members of a new family of antimicrobial peptides from the hemolymph of shrimps Penaeus vannamei in which immune response has not been experimentally induced. The three molecules display antimicrobial activity against fungi and bacteria with a predominant activity against Gram-positive bacteria. The complete sequences of these peptides were determined by a combination of enzymatic cleavages, Edman degradation, mass spectrometry, and cDNA cloning using a hemocyte cDNA library. The mature molecules (50 and 62 residues) are characterized by an NH2-terminal domain rich in proline residues and a COOH-terminal domain containing three intramolecular disulfide bridges. One of these molecules is post-translationally modified by... |
| Tipo: Text |
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| Ano: 1997 |
URL: http://archimer.ifremer.fr/doc/00335/44649/57344.pdf |
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| Destoumieux, Delphine; Munoz, Marcello; Cosseau, Céline; Rodriguez, Jenny; Bulet, Philippe; Comps, Marie-annick; Bachere, Evelyne. |
| Penaeidins are members of a new family of antimicrobial peptides isolated from a crustacean, which present both Gram-positive antibacterial and antifungal activities. We have studied the localization of synthesis and storage of penaeidins in the shrimp Penaeus vannamei. The distribution of penaeidin transcripts and peptides in various tissues reveals that penaeidins are constitutively synthesized and stored in the shrimp haemocytes. It was shown by immunocytochemistry, at both optical and ultrastructural levels, that the peptides are localized in granulocyte cytoplasmic granules, The expression and localization of penaeidins were further analysed in shrimp subjected to microbial challenge. We found that (1) penaeidin mRNA levels decrease in circulating... |
| Tipo: Text |
Palavras-chave: Crustacean immunity; Penaeid shrimp; Haemocyte; Chitin binding; Antimicrobial peptide. |
| Ano: 2000 |
URL: http://archimer.ifremer.fr/doc/2000/publication-412.pdf |
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